Biorad tgx precast gels plus#

Bis-Tris Gels use a Bis-Tris discontinuous buffer system involving three ions: The gels can be run using MES SDS running buffer to better resolve smaller proteins (1вЂ“200 kDa) and MOPS SDS running buffer to resolve medium- to large-size proteins (14вЂ“260 kDa). For optimal sample preparation, we recommend using buffers containing SDS.

Biorad tgx precast gels plus#

NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels are formulated for denaturing gel electrophoresis applications. High capacity wells of Bolt Bis-Tris Plus Gels-wedge-shaped wells makes sample loading easier (no specialized pipette tips required) and allow loads of up to two times higher sample volume.Longer shelf life-NuPAGE Bis-Tris and Bolt Bis-Tris Plus gels can be stored for at least 12 months and 16 months at room temperature, respectively.Faster run times-separation of your proteins can be attained in 22-35 minutes.Wide ranges of molecular weight separation-selecting the right gel and running buffer helps to achieve the optimal separation of your proteins.Better protein integrity-optimized sample preparation process preserves your proteins.Features of the NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels include: The unique WedgeWell design of the Bolt Bis-Tris Plus gel sample well allows loading of up to 2x more sample volume than other precast gels. Both NuPAGE Bis-Tris gels and Bolt Bis-Tris Plus gels are ideal for western blot analysis and applications such as sequencing and mass spectrometry where protein integrity is crucial and higher sensitivity is required. NuPAGE Bis-Tris gels are offered as mini and midi gels, and Bolt Bis-Tris Plus gels are offered as mini gels. Additionally, preserving protein integrity becomes particularly important when separating low-abundance proteins. The Bis-Tris gel chemistry provides a neutral-pH environment that minimizes protein modifications, resulting in sharper bands without typical gel 'smiling'. NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels are precast polyacrylamide gels designed to give optimal separation for a wide range of molecular weight proteins during gel electrophoresis under denaturing conditions. *Not all percentages are available in every well type WedgeWell format: 10, 12, 15 17 well (load up to 2X sample volume per well) SureLock Tandem Midi Gel Tank, Invitrogen XCell4В SureLockВ Midi-CellВ or Bio-Rad Criterion (with adapters only) Mini Gel TankВ or XCellВ SureLockВ Mini-Cell NuPAGE MES SDS or NuPAGE MOPS SDS Running BufferД«olt MES SDS or Bolt MOPS SDS running buffer The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.Working with dilute samples or need to load larger sample volumes, or when faster run times are neededД¬oefficient of variation (CV) of only 2% for Rf values (migration) The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations. Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. Mini-PROTEANВ®В TGX Stain-Freeв„ў Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins withВ Bio-Rad stain-free imaging systems.